Romana

Research project CNCSIS PN II / Idei - 326 / 2007

1. Project summary
2. Research team
3. Young researchers' implication
4. Objectives, activities, completion status
5. Results
6. Publications

1. Project summary (Romanian, English).

2. Research team
At the beginning of the research grant:

• Prof. Dr. Constanta Ganea (director)
• Dr. Irina Baran
• As. Drd. Anca Popescu (absent in 2008, due to post-doc research activities at Max-Planck Institute of Biophysics, Frankfurt/Main, Germany; she formally became a PhD in the meantime)
• As. Drd. Adrian Iftime (he became a PhD in the meantime)
• Ms.of.sc. candidate, Octavian Calinescu
  

3. Young researchers' implication:

There are some young researchers in-training involved in this grant: Anca Popescu, Adrian Iftime, Octavian Calinescu (see below the publication list)

• 2007: 50%
• 2008: 37% (absent in 2008, due to post-doc research activities at Max-Planck Institute of Biophysics, Frankfurt/Main, Germany, in a DFG partnership)
• 2009: 54%
• 2010: 54%

4. Objectives, activities, completion status:

• 2007
  • 1. Seminar - project presentation
    • 1.1. Seminar organized for all project participants; managerial and scientific tasks distribution, laboratory assessments.
    • 1.2. Project web-page, hosting, etc.
  • Completion status: total
    
    
  • 2. Design of scientific methods and laboratory protocols
    • 2.1. Design of several Ach diffusion models in synapses (in vivo) and in a patch-clamp setting
    • 2.2. Making of a preliminary patch-clamp measurements, about GAL effects on nicotinic receptors.
    • 2.3. Results analysis, seminar
    • 2.4. Full scientific report submission
  • Completion status: total
    
    
• 2008
  • 1. The study of GAL effects on nicotinic receptor. Knowledge enhancement.
    • 1.1. Patch-clamp setup operation instruction for student (univ. / master / doctoral)
    • 1.2. Patch-clamp measurements on the GAL effects at the ACh nicotinic receptor; institutional collaboration with Max-Planck Institute for Biophysics, Frankfurt/Main, Istituto Nazionale Catania
    • 1.3. Experimental data analysis, ; institutional collaboration Max-Planck Institute for Biophysics, Frankfurt/Main, Universidade Nova Lisabona
    • 1.4. Experimental setup development and upgrade, amplifiers, accessories, ice-flake machine
  • Completion status: total
    
    
  • 2. Results dissemination, increase of national and international research impact
    • 2.1. Publications - web, scientific conferences/ articles in scientific journals; joint seminar Frankfurt/Main , Catania/Italy
  • Completion status: total
    
    
  • 3. The study of GAL on artificial lipid membranes through BLM (Black Lipid Method). Knowledge enhancement.
    • 3.1. BLM set-up, laboratory protocols for electrophysiology; Laboratory preparation and inventory.
    • 3.2. BLM measurements on GAL effects on lipid membranes. Chemicals, reagents, laboratory preparations.
    • 3.3. Experimental data analysis.
    • 3.4. Theoretical model of GAL interaction with lipid membranes. Computer data systems.
  • Completion status: total
    
    
  • 4. Increase of national and international research impact
    • 4.1. Dissemination: scientific reports, web, articles conferences
    • 4.2. Scientific seminar - result analysis and project management status. Yearly report (final).
  • Completion status: total
    
    
• 2009
• 1.1. The study of GAL on artificial lipid membranes through SSM (Solid Supported Membranes method). Knowledge enhancement.
  • 1.1.1. SSM Measurements on GAL, collaboration with Max-Planck for Biophysics, Frankfurt/Main
  • 1.1.2. Experimental data analysis
• Completion status: total
  
  
• 1.2. The study of GKB on artificial lipid membranes through SSM (Solid Supported Membranes method). Knowledge enhancement.
  • 1.2.1. SSM Measurements on GKB and artificial lipid membranes, collaboration with Max-Planck for Biophysics, Frankfurt/Main
  • 1.2.2. Experimental data analysis, interpretation
  • 1.2.3. Increase of national and international research impact through scientific publications.
• Completion status: total
  
  
• 2.1. The study of GKB on artificial lipid membranes through BLM. Knowledge enhancement.
  • 2.1.1. BLM electrophysiology measurements on GKB - artificial lipid membrane interactions. Lab. chemicals.
  • 2.1.2. Experimental data analysis, interpretation
• Completion status: total
  
  
• 2.2. Preliminary studies on GKB interactions with nicotinic ACh receptor. Knowledge enhancement.
  • 2.2.1. Preliminary experimental measurements on GKB-ACh nicotinic receptor through patch-clamp method. Laboratory chemicals, apparatus (completion of spectrofluorimeter device)
  • 2.2.2. Experimental data analysis, interpretation
• Completion status: total
  
  
• 2.3. Increase of national and international research impact through scientific publications.
  • 2.3.1. Dissemination, scientific report - web, conference / article in scient. journal
  • 2.3.2. Internal self-evaluation at team level, auditing, yearly report.
• Completion status: total
  
  
• 2010
• 1. Creation of a GAL action model and subsequently improving the research on GKB ifluence on nicotinic receptor.
• 2. Information dissemniation through peer-reviewed publications  (5 published articles, 3 BDI and 2 ISI)
• 3. Analysis and syntesis of the project's results. Final raport (see below, #5)
  
• Completion status: total

 

 5. Results (detailed for each research grant time-slot):

• December 2007 (summary ro, eng)
  • Results (summary)
• Yearly report 2008 (31 oct.)
  • Results (summary)
• Report I -  2009
  • Results (summary)
• Report II - 2009
  • Results (summary)
• Yearly report 2010 (nov.)
  • Results (summary)
• Final report
  
  • Synthesis and final data analysis (summary)
    
    

6. Publications :

• I. Băran. Modulation of calcium signals by fluorescent dyes in the presence of tubular endoplasmic reticulum: a modelling approach. BioSystems 92(3):259-269 (2008)  [ISI, f.i.  1.477] (link ISI) (web-link)
• Anca Popescu, Irina Băran, Al. Morega, Constanta Ganea, Drug delivery in a patch-clamp setup: Computational models of concentration distribution on the cell membrane, Proceedings of the Seventh Workshop on Mathematical Modeling of Environmental and Life Sciences, Constanta, Romania, Ed. Academiei Romane, Sept. 2008 [BDI] (web-link)
  
• Anca Popescu, T. Moura, C. Ganea, A. Bicho, Galantamine modulates the recovery from desensitization of nicotinic receptors in TE 671 cells, Rom. Biotechnol. Letters, 2009, 14(1):4104-4118. [ISI, f.i. 0.33] (link ISI) (web-link)
• Irina Băran, Anca Popescu, A model-based method for estimating Ca2+ release fluxes from linescan images in Xenopus oocytes, Chaos, 2009, Sep;19(3):037106  [ISI, f.i. 2.197] (link_ISI)(web-link)
• A. Iftime, A. Popescu, C. Ganea, On the galantamine interaction with artificial lipid membranes, Rom. J. Biophys. 2010 20(2): 000-000 (BDI- indexat in Index Copernicus) (web-link)
• Irina Băran, Adrian Iftime, Anca Popescu, Diffusion–convection effects on drug distribution at the cell membrane level in a patch-clamp setup, Biosystems, 2010, 102: 134-137, [ISI ](web-link)(doi link).
• A.Iftime, A. Popescu, C. Ganea, On the mechanisms of galantamine interaction with artificial lipid bilayers, Romanian Biotechnological Letters, 2010, 15 Issue: 6, in printing. [ISI, f.i. 0.33].
• A. Popescu, A. Bicho, C. Ganea, Effects of Quercetin on Muscle-Type Nicotinic Receptor Activation and Desensitization, Romanian Biotechnological Letters, 2011, 16 (1), accepted [ISI, f.i. 0.33].
• A. Popescu, A. Iftime, C. Ganea, Ginkgolide B effects on artificial lipid membranes, Rom. J. Biophys. 2010 vol 20 no.4, pp 283-293 (BDI- indexat in Index Copernicus).
  

Last update: Nov. 2010
Note: this site is updated at the end of each grant time-slot completion and also whenever a publication, article or seminar arises. The structure of this page complies with the CNCSIS' guidelines (april 2010)