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Titlul proiectului : "Study on the molecular and cellular mechanisms of action of EGCG and quercetin as potential chemotherapeutic agents in human leukemia Jurkat T-cells"
(Studiul mecanismelor moleculare si celulare ale actiunii EGCG si quercetinei ca potentiali agenti chemoterapeutici pentru celulele leucemice umane Jurkat T)

Grant de cercetare numarul: 342/2011
Cod grant: PNII-IDEI-PCE-2011-3-0800
Acesta un grant de cercetare al Autoritatii Nationale pentru Cercetare Stiintifica, CNCS - UEFISCDI, Romania


Echipa proiectului :

  • Prof. Dr. Constanta Ganea - director de proiect
  • Conf. Dr. Irina Baran - cercetator principal
  • Conf. Dr. Diana Ionescu - cercetator postdoctoral
  • Sef lucr. Dr. Adrian Iftime - cercetator postdoctoral
  • Sef lucr. Dr. Magdalena Mocanu - cercetator postdoctoral
  • Rodica Zaharia - tehnician
  • Lenuta Constantin - laborant
  • Ioana Tofolean - student
  • Ruxandra Irimia - student
  • Alexandru Garaiman - student
Sumarul proiectului : (.pdf)
Obiective si activitati: (.pdf, aditional 2013)

Publicatii:
  • Articole:

    1. Ioana Teodora Tofolean, Constanta Ganea, Diana Ionescu, Alexandru Filippi, Alexandru Garaiman, Alexandru Goicea, Mihnea-Alexandru Gaman, Alexandru Dimancea, Irina Baran, Cellular determinants involving mitochondrial dysfunction, oxidative stress and apoptosis correlate with the synergic cytotoxicity of epigallocatechin-3-gallate and menadione in human leukemia Jurkat T cells, PHARMACOLOGICAL RESEARCH, DOI 10.1016/j.phrs.2015.12.013, Volume 103, January 2016 pp. 300-317, 2015 (if. 4.403)(is.1.17) (ISI)

    2. Irimia Ruxandra, Tofolean Ioana, Ganea Constanta, Baran Irina, Quercetin, Menadione, Doxorubicin combination as a potential alternative to Doxorubicin monotherapy of acute lymphoblastic leukemia, DOCUMENTA HAEMATOLOGICA, DOI: 10.1515/dch-2015-0005, vol. 34, 1-2, pp. 52-61, 2016, (PubMed, BDI)

    3. I. Baran, A. Scordino, D. Ionescu, S. Privitera, R. Grasso, M. Gulino, F. Musumeci, I.T. Tofolean, C. Ganea, Functional characterization of mitochondrial respiratory complex I by delayed luminescence spectroscopy, LNS ACTIVITY REPORT 2015, Istituto Nazionale Di Fisica Nucleare Laboratori Nazionali Del Sud, sub tipar, 2015; Edit. Marchese Arti Grafiche, Siracusa, Italia; ISSN: 1827-1561 (BDI)

    4. A. Iftime, C. Ganea, A. Popescu Rightmire, Interference of coumarin with the insertion of lyotropic anions and cadmium in artificial lipid bilayers, ROMANIAN BIOTECHNOLOGICAL LETTERS, Vol. 20, No. 4, Pages: 10637-10647, 2015 (if. 0.404)(is 0.115) (ISI)

    5. Agata Scordino, Irina Baran, Marisa Gulino, Constanta Ganea, Rosaria Grasso, J. Hugo Niggli,Francesco Musumeci, Ultra-weak Delayed Luminescence in cancer research: A review of the results by the ARETUSA equipment, JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B: BIOLOGY 139 , pp. 76-84 (2014)(IF 2.803) (is. 0.843) (ISI)

    6. Irina Baran, Eva Katona, Constanta Ganea, Quercetin as a fluorescent probe for the Ryanodine receptor activity in Jurkat cells, PFLUEGERS ARCHIV, EUROPEAN JOURNAL OF PHYSIOLOGY, 2013, DOI 10.1007/s00424-013-1235-y, 465 Issue: 8 Pages: 1101-1119. (IF 4.866) (is. 1.857) [link] [link] (ISI)

    7. Irina Baran, Constanta Ganea, RyR3 in situ regulation by Ca2+ and quercetin and the RyR3-mediated Ca2+ release flux in intact Jurkat cells, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 540 (2013) 145–159, [DOI: link], ISSN 0003-9861 [link] (IF. 3.043) (is. 1.285) (ISI)

    8. Baran, Irina; Ganea, Constanta; et al., "Detailed Analysis of Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T Cells after Proton Irradiation and Treatments with Oxidant Agents and Flavonoids", OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, Article Number: 498914, DOI: 10.1155/2012/498914, 2012 [link] (IF:3.393) (is 0.691) (ISI)

    9. Mocanu Maria-Magdalena, Surcel Mihaela, Ursaciuc Cornel, Katona Eva, Ganea Constanta, Antiproliferative effect of quercetin in mammary and epidermoid cancer, ROMANIAN BIOTECHNOLOGICAL LETTERS, 2013, vol. 18, no. 6, pp. 8796-8803,  (IF. 0.363)(is 0.115) [ link ] (ISI)

    10. Irina Baran, Diana Ionescu, Alexandru Filippi, Maria Magdalena Mocanu, Adrian Iftime, Ramona Babes, Ioana Teodora Tofolean, Ruxandra Irimia, Alexandru Goicea, Valentin Popescu, Alexandru Dimancea, Andrei Neagu, Constanta Ganea, Novel insights into the antiproliferative effect of quercetin, menadione and rotenone in human leukemia Jurkat T cells, LEUKEMIA RESEARCH, 2014, Volume: 38 Issue: 7 Pages: 836-849, 2014 (IF 2.692) (is. 0.950) (ISI)

    11. Irina Baran, Diana Ionescu, Simona Privitera, Agata Scordino, Maria Magdalena Mocanu, Francesco Musumeci, Rosaria Grasso, Marisa Gulino, Adrian Iftime, Ioana Teodora Tofolean, Alexandru Garaiman, Alexandru Goicea, Ruxandra Irimia, Alexandru Dimancea, Constanta Ganea, Mitochondrial respiratory Complex I probed by delayed luminescence spectroscopy, JOURNAL OF BIOMEDICAL OPTICS, 2013, 18 (12), 127006, DOI: 10.1117/1.JBO.18.12.127006, (IF 2.752) (is. 1.910) [ link ] (ISI)

    12. M.M. Mocanu, C. Ganea, L. Georgescu, T. Varadi, D. Shrestha, I. Baran, E. Katona, P. Nagy, J. Szöllösi, Epigallocatechin-gallate determines ErbB proteins downregulation, cell death mediated by 67kDa laminin receptor and altered lipid order in mammary and epidermoid carcinoma cells, 2013, J. NAT. PROD., Volume: 77 Issue: 2, Pages: 250-257, 2014, DOI: 10.1021/np4007712, (IF. 3.947) (is. 2.158) [ link ] (ISI)

    13. Agata Scordino, Irina Baran, Marisa Gulino, Constanta Ganea, Rosaria Grasso, J. Hugo Niggli, Francesco Musumeci, Ultra-weak Delayed Luminescence in cancer research: A review of the results by the ARETUSA equipment, JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B: Biology, Vol. 139, pp. 76-84, 2014,  (IF 2.803) (is. 0.843) [ link ] (ISI)

  • Conferinte stiintifice cu comitet de program:

    1. Baran, I., Katona E., Ganea C., Quercetin fluorescence reveals the open probability of the RyR3 Ca2+ channel in intact cells, 22nd IUBMB Congress/37th FEBS, Seville, SPAIN,  SEP 04-09, 2012,  FEBS JOURNAL  Volume: 279   Special Issue: SI Suppl: 1, p: 269-270,  SEP 2011

    2. Ganea C., Katona E., Baran I., Quercetin activates the RyR3 receptor in Jurkat human leukemia cells, invitatie la conferinta Institutului Max-Planck de Biofizica, Frankfurt/Main, Germania, 28.10. 2011.

    3. Agata Scordino, I. Baran, V. Baran, R. Bonfanti, A. Campisi, C. Ganea, R. Grasso, F. Musumeci, R. Parenti - New insights into application of Delayed Luminescence in cancer research - Photodynamic Therapy and Photodiagnosis in Clinical Practice: 9th International Symposium 2012 (October 16-20, 2012 - Brixen / Bressanone, Italy)

    4. Ionescu D., Ganea C.,  Iftime A., Tofolean I.T., Irimia R., Goicea A., Dimancea A., Neagu A., Baran I., Quercetin exerts its antitumoral effect while manifesting a strong antioxidant character, 9th European Biophysics Congress - EBSA 2013, Lisabona, Portugalia (acceptat),  Abstract Booklet, Eur. Biophys. J., (2013), P52, 42: S35=S208, S48, 2013

    5. M.M. Mocanu, P. Nagy, L. Georgescu, T. Varadi, D. Shrestha, I. Baran, E. Katona,  J. Szollosi, C. Ganea, The effect of flavonoids in mammary and epidermoid tumor cells with ErbB proteins overexpression, 8th International Conference "Structure and Stability of Biomacromolecules - SSB 2013", 2013, Kosice, Slovakia

    6. D. Ionescu, The influence of quercetin on lipid membranes with cholesterol, 8th International Conference "Structure and Stability of Biomacromolecules - SSB 2013", 2013, Kosice, Slovakia

    7. Agata Scordino, Irina Baran, Roberta Bonfanti, Agata Campisi, Costanta Ganea, Rosaria Grasso, Marisa Gulino, Adrian Iftime, Diana Ionescu, Maria Magdalena Mocanu, Francesco Musumeci, Rosalba Parenti - Delayed Luminescence spectroscopy to monitor mitochondrially targeted effects of cell proliferation inhibitors - Ultra-weak Photon Emission From Living Systems Conference (June 21-23, 2013, Olomouc, Czech Republic) - Abstract in press on Journal of Acupuncture and Meridian Studies

    8. Agata Scordino, I. Baran, R. Bonfanti, A. Campisi, C. Ganea, R. Grasso, M. Gulino, A. Iftime, D. Ionescu, M. M. Mocanua, F. Musumeci, R. Parenti - Delayed Luminescence spectroscopy in cancer research - XCIX Congresso Nazionale Societa' Italiana di Fisica (23 - 27 settembre 2013, Trieste, Italy) – Book of Abstracts p. 120.

    9. R.G. Sandu, I. Baran, D. Ionescu, A. Filippi, R. Babes, A. Goicea, A. Garaiman, R. Irimia, A. Dimancea, M. Gaman, C. Ganea, A. Murgoci. Doxorubicin induces G2/M arrest, Ca2+ release, oxidative stress and apoptosis in human leukemia Jurkat cells at clinical doses and dose rates, poster (acceptat), FEBS EMBO 2014 Congress, 30 August - 4 Septembrie 2014, Paris, Franta. Rezumatul va fi publicat în FEBS Journal, ISSN: 1742-464X; revistă cotată ISI; sub tipar

    10. M.M. Mocanu, T. Picot, E. Radu, I. Baran, E. Katona, P. Nagy, J. Szöllősi, L. Campos, C. Ganea. Modulation of motility and signaling pathways by epigallocatechin-3-O-gallate in tumor cell lines overexpressing ErbB proteins, poster (acceptat), FEBS EMBO 2014 Congress, 30 Aug. - 4 Sept. 2014, Paris, Franta. Rezumatul va fi publicat în FEBS Journal, ISSN: 1742-464X; revistă cotată ISI; sub tipar

    11. Alexandru Garaiman, Diana Ionescu, Alexandru Filippi, Ramona Babes, Alexandru Goicea, Valentin Popescu, Ruxandra Irimia, Alexandru Dimancea, Mihnea Gaman, Adrian Murgoci, Constanta Ganea, Irina Baran. Synergic antiproliferative effect of Epigallocatechine-3-gallate and menadione in human leukemia Jurkat T cells. Poster, 7th EFIS/EJI South East European Immunology School, Timişoara, 26-29 sept. 2014

    12. Mihnea-Alexandru Gaman, Alexandru Filippi, Diana Ionescu, Constanta Ganea, Irina Baran, Modulation of the antitumoral effect of doxorubicin by quercetin-menadione combinations in human leukemia Jurkat cells, Craiova International Medical Students Conference (6-9th November, 2014)

    13. Valentin Popescu, Bogdan Mastalier, Irina Baran. Polycaprolactone microspheres loaded with bioflavonoids and menadione mixture, novel systemic-friendly approach on cancer. Prezentare orală acceptată la workshop-ul international „Electroporation based Technologies and Treatments” organizat de European Society for Biomaterials, Ljubljana, Slovenia, 15-21 nov. 2015

    14. Roxana Gabriela Sandu, Ioana Teodora Tofolean, Constanta Ganea, Irina Baran. Enhancement of the antiproliferative effect of doxorubicin by quercetin-menadione combinations in human leukemia Jurkat cells. Poster, 6th EMBO (European Molecular Biology Organization) Meeting, 5-8 sept. 2015, Birmingham, Marea Britanie

    15. Alexandru Garaiman, Ioana Teodora Tofolean, Irina Baran. Chemotherapeutic potential of combination EGCG:MD in human leukemia Jurkat T cells. Poster, 5th Lower Saxony International Summer Academy in Immunology, 16 august – 13 september 2015, Hannover Medical School, Hannover, Germania. Abstract Book pag. 3

    16. Oana Elena Baran, Ioana Teodora Tofolean, Ruxandra Irimia, Irina Baran, Constanta Ganea. Antiproliferative effect of doxorubicin/quercetin/menadione combination in leukemia Jurkat T cells. Poster, 10th EBSA European Biophysics Congress, 18-22 July 2015, Dresda, Germania. Late Abstract Booklet p. 44

    17. Vlad Cosoreanu, Ioana Teodora Tofolean, Constanta Ganea, Alexandru Goicea, Irina Baran. Growth-suppressive action of doxorubicin on human leukemia Jurkat cells. Modulation by quercetin. Poster, 10th EBSA European Biophysics Congress, 18-22 July 2015, Dresda, Germania. Late Abstract Booklet p. 45

    18. Maria Teodora Ilie, Ioana Teodora Tofolean, Constanta Ganea, Alexandru Dimancea, Irina Baran. Chemotherapeutic potential of the doxorubicin/menadione combination in a human leukemia cell model. Poster, 10th EBSA European Biophysics Congress, 18-22 July 2015, Dresda, Germania. Late Abstract Booklet p. 45

    19. Valentin Popescu, Bogdan Mastalier, Irina Baran. Bioflavonoids and menadione potential in novel systemic-friendly approach on cancer. Poster la workshop-ul international „YOUNG SCIENTISTS JOINING FORCES FOR EXCELLENCE IN BIOMATERIALS RESEARCH” organizat de European Society for Biomaterials, Bucureşti, 28-29 mai 2015. Lucrare distinsă cu premiul „Best Poster Award”

    20. Ruxandra Irimia, Irina Baran. Green tea derived EGCG and vitamin K3: a synergic antiproliferative effect in acute lymphoblastic leukemia. MEDICALIS International Congress for Medical Students and Young Professionals, 14-17 May 2015, Cluj-Napoca

    21. Paul Ciucur, Simona Costache, Irina Baran, Constantea Ganea. Possible therapeutic attitude towards acute lymphoblastic leukemia. MEDICALIS International Congress for Medical Students and Young Professionals, 14-17 May 2015, Cluj-Napoca

    22. Simona Costache, Paul Ciucur, Alexandru Filippi, Irina Baran. Modularea efectului antitumoral al doxorubicinei asupra celulelor umane leucemice Jurkat cu combinații quercetină-menadionă. Comunicare orală, Congresul Naţional pentru Studenţi şi Tineri Medici, Bucureşti, martie 2015. Lucrare distinsă cu Premiul I. Carte de Abstracte ISSN 2285-9438

    23. A. Filippi, T. Picot, C.M. Aanei, L. Campos, C. Ganea, M.M. Mocanu, Epigallocatechin-3-O-gallate reduces the clonogenicity, induces the mitochondrial depolarization and increases production of reactive oxygen species in cancer cell lines with ErbB proteins overexpression, International Conference of the Romanian Society of Biochemistry and Molecular Biology, Bucharest, 17-18 September 2015, ISBN 978-973-720-605-3

    24. M.M. Mocanu, A. Filippi, T. Picot, C. M. Aanei, L. Campos, C. Ganea, Anticancer effects of epigallocatechin-3-O-gallate in tumor cell lines with ErbB proteins overexpression, poster, National Conference of Cytometry, May 20, 2015, Bucharest, Romania

    25. Oana Elena Baran, Ioana Teodora Tofolean, Maria Catalina Ceausescu, Constanta Ganea, Analysis of convergent effects of doxorubicin, quercetin and menadione in treatments of human leukemia Jurkat T cells, 12th World Cancer Conference, September 26-28, 2016 London, UK, Abstracts, p. 78, ISSN: 1948-5956

    26. Vlad Cosoreanu, Ioana Teodora Tofolean, Ramona Babes, Irina Baran, Cytotoxic effects of doxorubicin on human leukemia Jurkat T cells. Drug interactions with quercetin, 12th World Cancer Conference, September 26-28, 2016 London, UK, Abstracts, p. 79, ISSN: 1948-5956

    27. Maria Teodora Ilie, Ioana Teodora Tofolean, Ramona Babes, Irina Baran, Cytotoxic effect of doxorubicin/menadione combination on human leukemia Jurkat cells, 12th World Cancer Conference, September 26-28, 2016 London, UK, Abstracts, p. 80, ISSN: 1948-5956

    28. Oana-Alina Ciolac, Alexandru Filippi, Constanta Ganea, Maria-Magdalena Mocanu, The effect of curcumin on epidermoid carcinoma cell line A-431, Congresul Universitatii de Medicina si Farmacie Carol Davila, Editia a 4-a, 2-4 iunie 2016, Bucuresti, Romania 

    29. Alexandru Filippi, Constanta Ganea, Maria-Magdalena Mocanu, Anti-proliferative effect of curcumin in ER+ and ErbB2+ mammary cancer cell lines, Congresul Universitatii de Medicina si Farmacie Carol Davila, Editia a 4-a, 2-4 iunie 2016, Bucuresti, Romania 

    30. Alexandru Filippi, Tiphanie Picot, Carmen Mariana Aanei, Lydia Campos, Constanta Ganea, Maria-Magdalena Mocanu, EGCG reduces partially the expression level of intracellular phosphorylated proteins in correlation with induction of cell death in epidermoid and mammary cancer cell lines, Congresul Universitatii de Medicina si Farmacie Carol Davila, Editia a 4-a, 2-4 iunie 2016, Bucuresti, Romania

  • Distinctii stiintifice:

    1. Key scientific article (Quercetin as a fluorescent probe for the ryanodine receptor activity in Jurkat cells, Baran I, Katona E, Ganea C., Pflugers Arch. 2013 Mar 9.), in Global Medical Discovery [link]

    2. Conferinta Modulation of the antitumoral effect of doxorubicin by quercetin-menadione combinations in human leukemia Jurkat cells a obtinut premiul II la Craiova International Medical Students Conference (6-9th November, 2014)

    3. Articolele 5, 6, 9, 10, 11 au fost premiate de UEFISCDI
Rapoarte de activitate stiintifica :



Contact
Prof. Dr. Constanta Ganea
Catedra de Biofizica Medicala
Universitatea de Medicina si Farmacie "Carol Davila"
Bd. Eroii Sanitari nr. 8,
050474 Bucuresti
Tel/Fax: 0040-21-3125955
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